The crash occurred when a tour bus carrying the group of Italian tourists rear-ended a stationary truck carrying paper towels on the motorway between Grimmenstein and Seebenstein around 12pm.
It is thought 32 people were left with minor injuries, eight with severe injuries and at least two were severely injured.
Although Austrian media reported yesterday that around 42 people were injured in the incident, the Italian Foreign Ministry confirmed last night that 46 Italians were among those injured.
Around 30 emergency vehicles and three helicopters took part in the rescue operation.
The helicopters were used to bring the two most critically injured people to bigger hospitals, Wiener Neustadt hospital and UKH Wien-Meidling hospital in Vienna.
The charter bus was carrying passengers between the ages of 40 to 50 and was nearly at full capacity when it crashed into the back of a truck.
The tour guide was thrown out of the vehicle during the coalition with the truck and was among the people who were most severely injured, according to the fire department.
With the help of the Red Cross and Austrian rescue services ÖAMTC, emergency services were able to bring all the injured people to nearby hospitals.
The south motorway was closed down around the area of the bus crash until approximately 5.30 pm in the direction of Vienna.
The rescue and clean-up operation took several hours, due to the large amounts of injured people and the fact that both vehicles were destroyed in the process of the crash.
Written by: Helena Uhl