Four fines for begging were issued last week, and in one case a lawyer has promised to help fight the fine.
Beggars who ask for money in parts of the city centre and at markets now face a €100 fine if caught by police. They then have two weeks to appeal the fine.
Plattform für Menschenrechte has set up a legal aid fund for beggars and will take their case to Salzburg's Administrative Court, and then on to the Constitutional Court.
A spokesman for the group, Josef Mautner, said that he is confident that the appeal will be successful and that the ban violates a basic human right.
The begging ban in Salzburg came into effect on June 1st after being voted in by the City Council.
At peak times, around 150 beggars per day are active in the centre of Salzburg. Munich, which has a population around ten times that of Salzburg, has a partial begging ban which has reduced the number of beggars in the city to around 50.